See the full transcripts,
cell by cell
The ArgenTag Single-Cell RNA Library Kit converts mRNA from individual cells into full-length, barcoded cDNA ready for PacBio or Oxford Nanopore sequencing.
No partitioning instrument.
No short-read inclusion list.
No isoform collapse.
VISION
Our solution
A complete benchtop workflow for single-cell RNA library prep on long-read sequencers. Cells are partitioned in a disposable microwell chip — loaded with a P200 pipette, no capital equipment — then barcoded with magnetic beads carrying ArgenTag's mathematically designed barcodes. Sample to cDNA in five hours, under two hours hands-on. Up to 20,000 cells per experiment. Our barcodes enable fast and reliable assignment of reads without requiring an inclusion list or additional short-read sequencing.
Compatible with PacBio Kinnex and Oxford Nanopore LSK 114.
OUR PRODUCT
Why ArgenTag?
Chip loads with a P200 at the bench
No short-read confirmation pass required
Isoforms, fusions, splicing, allele-specific expression, TCR/BCR/HLA
Gentle gravity partitioning, viable on sensitive samples
Compatible with PacBio, Oxford Nanopore Technologies and Illumina sequencing
OUR PRODUCT
From cells to sequenced isoforms in a day
Five hours from sample to cDNA. Under two hours of hands-on time.
No partitioning instrument, no inclusion list sequencing pass, no specialized training.
Step 1: Load
the chip
Pipette your cell suspension onto the ArgenTag microwell chip. Cells will settle into individual microwells by gravity.
Step 2: Capture
and barcode
Add the barcoded magnetic beads to pair with the cells in microwells. Lyse the cells in place, and recover the beads with the poly-A mRNA.
Step 3: Reverse transcribe and amplify
Perform the reverse transcription with the magnetic beads, and amplify to generate full-length, barcoded cDNA.
Step 4: Prepare your cDNA and sequence
Integrate the cDNA into your preferred long-read workflow using PacBio Kinnex or Oxford Nanopore LSK114, and proceed with sequencing.
Step 5: Demultiplex
and analyze
Process the raw sequencing data without barcode inclusion list: run ArgenTag's pipeline on your servers or upload your data and receive a report.
KOL and Early Adopter Testimonials
The cDNAs are quite long. This makes me much more hopeful about the quality of the isoforms from your platform compared to the standard.
Mount Sinai Icahn School of Medicine
❝
The barcode detection efficiency of your kits is excellent.
I believe there is no match to it.
Excellent high quality results!
Bulgarian Academy of Sciences
❝
It's an open system, not a black box. I could see my cells and track the process.
It allows my team to try new things and avoids unnecessary costs.
NYU Langone Health
❝
The process was straightforward and fast. I was able to complete the protocol in less time than expected.
The instructions were easy to follow.
CZ Biohub
❝
Contact us at welcome@argentag.com for more information or fill the following form
FAQs
